Extraction, Separation, and Structural Analysis of Ellagitannin in Strawberries
Poster Number
54
Format
Poster Presentation
Faculty Mentor Name
Andreas Franz
Faculty Mentor Department
Chemistry
Abstract/Artist Statement
Polyphenolic compounds including flavonoids and anthocyanins have been reported as antioxidants improving human health.1 These compounds are present in many fruits and vegetables and prevent cell damage from reactive oxygen species (oxidative stress). Methods previously reported by us were used to extract polyphenols from strawberries. Strawberry homogenate was subjected to Soxhlet extraction with methanol. After partitioning of the evaporated extract between water and chloroform followed by water and ethylacetate, compounds in the aqueous phase were adsorbed onto an Amberlite XAD-16 column, washed with water, and eluted with methanol. Significant amounts of bis-galloyl-HHDP glucose (ellagitannin), a well-known natural product with multiple beneficial medical properties, were detected in the ethylacetate extract. Further purification of ellagitannin was accomplished by reverse-phase C18-HPLC. Complete structural identification was made based on MALDI-TOF mass spectral analysis and NMR analysis.
1) Silva, J.P.; Coutinho, O.P. Drug Disc & Therap 2010. 4, 144-176
Location
DeRosa University Center, Ballroom
Start Date
20-4-2013 1:00 PM
End Date
20-4-2013 3:00 PM
Extraction, Separation, and Structural Analysis of Ellagitannin in Strawberries
DeRosa University Center, Ballroom
Polyphenolic compounds including flavonoids and anthocyanins have been reported as antioxidants improving human health.1 These compounds are present in many fruits and vegetables and prevent cell damage from reactive oxygen species (oxidative stress). Methods previously reported by us were used to extract polyphenols from strawberries. Strawberry homogenate was subjected to Soxhlet extraction with methanol. After partitioning of the evaporated extract between water and chloroform followed by water and ethylacetate, compounds in the aqueous phase were adsorbed onto an Amberlite XAD-16 column, washed with water, and eluted with methanol. Significant amounts of bis-galloyl-HHDP glucose (ellagitannin), a well-known natural product with multiple beneficial medical properties, were detected in the ethylacetate extract. Further purification of ellagitannin was accomplished by reverse-phase C18-HPLC. Complete structural identification was made based on MALDI-TOF mass spectral analysis and NMR analysis.
1) Silva, J.P.; Coutinho, O.P. Drug Disc & Therap 2010. 4, 144-176