Title

Isolation and Characterization of Cyanin Chloride from Strawberrie

Poster Number

70

Lead Author Major

Biochemistry

Format

Poster Presentation

Faculty Mentor Name

Andreas Franz

Faculty Mentor Department

Chemistry

Abstract/Artist Statement

Strawberries are convenient sources of polyphenolic compounds that have documented ability to act as radical scavengers and hence might be of general benefit to human health. We have reproduced a published method for the isolation of one specific poylphenolic compound, cyanin chloride; a red-colored pigment. Strawberries were homogenized and were subjected to Soxhlet extraction (methanol). The evaporated extract was redissolved in water and was partitioned between several organic solvents. The fractions were analyzed by Thin Layer Chromatography and Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF-MS). A commercially available cyanin chloride standard was used for reference, and its structure was confirmed by extensive NMR spectroscopy. The identity of the commercial standard and the extracted cyanin chloride was confirmed.

Location

Grave Covell

Start Date

21-4-2012 10:00 AM

End Date

21-4-2012 12:00 PM

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Apr 21st, 10:00 AM Apr 21st, 12:00 PM

Isolation and Characterization of Cyanin Chloride from Strawberrie

Grave Covell

Strawberries are convenient sources of polyphenolic compounds that have documented ability to act as radical scavengers and hence might be of general benefit to human health. We have reproduced a published method for the isolation of one specific poylphenolic compound, cyanin chloride; a red-colored pigment. Strawberries were homogenized and were subjected to Soxhlet extraction (methanol). The evaporated extract was redissolved in water and was partitioned between several organic solvents. The fractions were analyzed by Thin Layer Chromatography and Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF-MS). A commercially available cyanin chloride standard was used for reference, and its structure was confirmed by extensive NMR spectroscopy. The identity of the commercial standard and the extracted cyanin chloride was confirmed.