Effects of MAT alpha deletions on protein secretion in Pichia pastoris
Poster Number
57
Format
Poster Presentation
Faculty Mentor Name
Geoff Lin-Cereghino
Faculty Mentor Department
Biological Sciences
Additional Faculty Mentor Name
Joan Lin-Cereghino
Abstract/Artist Statement
Pichia pastoris is a yeast known to efficiently express and secrete heterologous proteins. In this yeast, MAT alpha is a signal that can direct protein secretion. Its effect on secretion can be tested using reporter genes, whose protein products can readily be measured. The goal of our project was to create specific deletions in the MAT alpha secretion signal and to determine their effects on secretion. Mutant constructs were made via site-directed mutagenesis, and the reporter genes tested were horseradish peroxidase (HRP) and lipase. We hypothesized that distinct deletions in the MAT alpha secretion signal should affect protein secretion differently, and these effects were tested by comparing secretion of the HRP and lipase proteins.
Location
Grave Covell
Start Date
21-4-2012 10:00 AM
End Date
21-4-2012 12:00 PM
Effects of MAT alpha deletions on protein secretion in Pichia pastoris
Grave Covell
Pichia pastoris is a yeast known to efficiently express and secrete heterologous proteins. In this yeast, MAT alpha is a signal that can direct protein secretion. Its effect on secretion can be tested using reporter genes, whose protein products can readily be measured. The goal of our project was to create specific deletions in the MAT alpha secretion signal and to determine their effects on secretion. Mutant constructs were made via site-directed mutagenesis, and the reporter genes tested were horseradish peroxidase (HRP) and lipase. We hypothesized that distinct deletions in the MAT alpha secretion signal should affect protein secretion differently, and these effects were tested by comparing secretion of the HRP and lipase proteins.