Title

In vitro Effect of Sanguinarine and Chelerythrine on Trichomonas vaginalis Trophozoites

Poster Number

24

Format

Poster Presentation

Abstract/Artist Statement

Trichomonas vaginalis is a parasitic protozoan that causes the STD trichomoniasis showing physiological symptoms in women and asymptomatic symptoms in men. To date, there are two FDA approved treatments for this disease, metronidazole and tinidazole. Currently, patient strains are becoming increasingly resistant and implements for new drug targets are needed. In this study, sanguinarine (SANG) and chelerythrine (CHE) were analyzed as additional chemotherapeutic treatments for trichomoniasis. This experiment aimed to assess the inhibitory effects of SANG and CHE on human trichomonad strains T1 and G3. Multiple drug assays with varying concentrations of SANG and CHE were performed to estimate the IC50, the concentration of the drug that would inhibit the growth of the parasites by 50%. The cell lines were tested in three environmental conditions: aerobic, anaerobic, and anaerobic with no ascorbic acid. Ascorbic acid is an antioxidant with proposed negative effects on SANG and CHE potency. Its activity was used to help discover the SANG and CHE mechanism. Cells were incubated for 24 hours (assay counts +/- 1 hr.) at 37C. IC50 molarities were skewed towards a narrow degree ranging from 1-10M exemplifying the potency. The data showed a trend of decreasing percent survival parallel to the increase of concentration of SANG and CHE. Previous data showed G3 strains were slightly more virulent than T1. In aerobic and anaerobic conditions, T1 had higher survival rates than G3. In anaerobic no ascorbic acid conditions, T1 generally had less survival than G3. The absence of ascorbic acid demonstrated increased SANG and CHE potency. This experiment utilized very low concentrations of SANG and CHE with high effectiveness. The data supports the hypothesis that these are possible drugs to go further in analysis as alternative forms of treatment.

Location

DeRosa University Center, Ballroom B

Start Date

2-5-2009 1:00 PM

End Date

2-5-2009 3:00 PM

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May 2nd, 1:00 PM May 2nd, 3:00 PM

In vitro Effect of Sanguinarine and Chelerythrine on Trichomonas vaginalis Trophozoites

DeRosa University Center, Ballroom B

Trichomonas vaginalis is a parasitic protozoan that causes the STD trichomoniasis showing physiological symptoms in women and asymptomatic symptoms in men. To date, there are two FDA approved treatments for this disease, metronidazole and tinidazole. Currently, patient strains are becoming increasingly resistant and implements for new drug targets are needed. In this study, sanguinarine (SANG) and chelerythrine (CHE) were analyzed as additional chemotherapeutic treatments for trichomoniasis. This experiment aimed to assess the inhibitory effects of SANG and CHE on human trichomonad strains T1 and G3. Multiple drug assays with varying concentrations of SANG and CHE were performed to estimate the IC50, the concentration of the drug that would inhibit the growth of the parasites by 50%. The cell lines were tested in three environmental conditions: aerobic, anaerobic, and anaerobic with no ascorbic acid. Ascorbic acid is an antioxidant with proposed negative effects on SANG and CHE potency. Its activity was used to help discover the SANG and CHE mechanism. Cells were incubated for 24 hours (assay counts +/- 1 hr.) at 37C. IC50 molarities were skewed towards a narrow degree ranging from 1-10M exemplifying the potency. The data showed a trend of decreasing percent survival parallel to the increase of concentration of SANG and CHE. Previous data showed G3 strains were slightly more virulent than T1. In aerobic and anaerobic conditions, T1 had higher survival rates than G3. In anaerobic no ascorbic acid conditions, T1 generally had less survival than G3. The absence of ascorbic acid demonstrated increased SANG and CHE potency. This experiment utilized very low concentrations of SANG and CHE with high effectiveness. The data supports the hypothesis that these are possible drugs to go further in analysis as alternative forms of treatment.