Title

Design of a Magnetic Liposome Drug Delivery System

Format

SOECS Senior Project Demonstration

Abstract/Artist Statement

In tumor treatments using liposomes, the biggest challenge is initiating the maximum release of the encapsulated drug at the tumor site. This anti-tumor treatment uses a novel drug delivery system which utilizes the abilities of liposomes to encapsulate various substances, in this case, magnetites, to localize the delivery of the drug. The liposome have been created using DPPC (DPPC–1,2-Dipalmitoyl-sn-Glyecro-3-Phosphocholine), DSPE-PEG-2000 (1,2-Distearoyl-sn- Glycero-3-Phosphoethanolamine-N-[Methoxy (Polyethylene glycol)-2000]), and MPPC (1- Myristoyl-2-Palmitoyl-sn-Glycero-3-Phosphocholine), which lowers the phase transition temperature of the liposomes. In this project, fluorescent ANTS/DPX dye is encapsulated to determine the permeability of the liposome membrane. Encapsulated magnetites reduced the amount of microwave energy needed to disrupt the membranes. A 1000W microwave was used to irradiate the liposome batches at 100% power for 4 seconds. Testing consisted of comparing the fluorescent intensity using a spectrofluorophotometer. The data collected was used to find the amount of encapsulated dye released after each treatment. All liposome batches were given the same treatments: No microwave heating, microwave heating (4s. at 100% Power), and the addition of detergent. When compared to the negative control liposomes (DPPC + DSPE-PEG 2000), the inclusion of MPPC and magnetites increased the amount of encapsulated dye released after microwave heating.

Location

School of Engineering and Computer Sciences

Start Date

5-5-2007 2:00 PM

End Date

5-5-2007 3:30 PM

This document is currently not available here.

Share

COinS
 
May 5th, 2:00 PM May 5th, 3:30 PM

Design of a Magnetic Liposome Drug Delivery System

School of Engineering and Computer Sciences

In tumor treatments using liposomes, the biggest challenge is initiating the maximum release of the encapsulated drug at the tumor site. This anti-tumor treatment uses a novel drug delivery system which utilizes the abilities of liposomes to encapsulate various substances, in this case, magnetites, to localize the delivery of the drug. The liposome have been created using DPPC (DPPC–1,2-Dipalmitoyl-sn-Glyecro-3-Phosphocholine), DSPE-PEG-2000 (1,2-Distearoyl-sn- Glycero-3-Phosphoethanolamine-N-[Methoxy (Polyethylene glycol)-2000]), and MPPC (1- Myristoyl-2-Palmitoyl-sn-Glycero-3-Phosphocholine), which lowers the phase transition temperature of the liposomes. In this project, fluorescent ANTS/DPX dye is encapsulated to determine the permeability of the liposome membrane. Encapsulated magnetites reduced the amount of microwave energy needed to disrupt the membranes. A 1000W microwave was used to irradiate the liposome batches at 100% power for 4 seconds. Testing consisted of comparing the fluorescent intensity using a spectrofluorophotometer. The data collected was used to find the amount of encapsulated dye released after each treatment. All liposome batches were given the same treatments: No microwave heating, microwave heating (4s. at 100% Power), and the addition of detergent. When compared to the negative control liposomes (DPPC + DSPE-PEG 2000), the inclusion of MPPC and magnetites increased the amount of encapsulated dye released after microwave heating.