Design of a Magnetic Liposome Drug Delivery System
Format
SOECS Senior Project Demonstration
Abstract/Artist Statement
In tumor treatments using liposomes, the biggest challenge is initiating the maximum release of the encapsulated drug at the tumor site. This anti-tumor treatment uses a novel drug delivery system which utilizes the abilities of liposomes to encapsulate various substances, in this case, magnetites, to localize the delivery of the drug. The liposome have been created using DPPC (DPPC–1,2-Dipalmitoyl-sn-Glyecro-3-Phosphocholine), DSPE-PEG-2000 (1,2-Distearoyl-sn- Glycero-3-Phosphoethanolamine-N-[Methoxy (Polyethylene glycol)-2000]), and MPPC (1- Myristoyl-2-Palmitoyl-sn-Glycero-3-Phosphocholine), which lowers the phase transition temperature of the liposomes. In this project, fluorescent ANTS/DPX dye is encapsulated to determine the permeability of the liposome membrane. Encapsulated magnetites reduced the amount of microwave energy needed to disrupt the membranes. A 1000W microwave was used to irradiate the liposome batches at 100% power for 4 seconds. Testing consisted of comparing the fluorescent intensity using a spectrofluorophotometer. The data collected was used to find the amount of encapsulated dye released after each treatment. All liposome batches were given the same treatments: No microwave heating, microwave heating (4s. at 100% Power), and the addition of detergent. When compared to the negative control liposomes (DPPC + DSPE-PEG 2000), the inclusion of MPPC and magnetites increased the amount of encapsulated dye released after microwave heating.
Location
School of Engineering and Computer Sciences
Start Date
5-5-2007 2:00 PM
End Date
5-5-2007 3:30 PM
Design of a Magnetic Liposome Drug Delivery System
School of Engineering and Computer Sciences
In tumor treatments using liposomes, the biggest challenge is initiating the maximum release of the encapsulated drug at the tumor site. This anti-tumor treatment uses a novel drug delivery system which utilizes the abilities of liposomes to encapsulate various substances, in this case, magnetites, to localize the delivery of the drug. The liposome have been created using DPPC (DPPC–1,2-Dipalmitoyl-sn-Glyecro-3-Phosphocholine), DSPE-PEG-2000 (1,2-Distearoyl-sn- Glycero-3-Phosphoethanolamine-N-[Methoxy (Polyethylene glycol)-2000]), and MPPC (1- Myristoyl-2-Palmitoyl-sn-Glycero-3-Phosphocholine), which lowers the phase transition temperature of the liposomes. In this project, fluorescent ANTS/DPX dye is encapsulated to determine the permeability of the liposome membrane. Encapsulated magnetites reduced the amount of microwave energy needed to disrupt the membranes. A 1000W microwave was used to irradiate the liposome batches at 100% power for 4 seconds. Testing consisted of comparing the fluorescent intensity using a spectrofluorophotometer. The data collected was used to find the amount of encapsulated dye released after each treatment. All liposome batches were given the same treatments: No microwave heating, microwave heating (4s. at 100% Power), and the addition of detergent. When compared to the negative control liposomes (DPPC + DSPE-PEG 2000), the inclusion of MPPC and magnetites increased the amount of encapsulated dye released after microwave heating.