Synthesis and Evaluation of the Peptide LEV
Poster Number
14
Format
Poster Presentation
Abstract/Artist Statement
The tri-peptide Leu-Glu-Val (LEV) has a structure similar to that of Leu-Asp-Val (LDV). Studies indicate that peptides containing LDV have potential to serve as a target specific drug delivery carriers. LEV was synthesized and its gas phase fragmentation property was evaluated as compared to LDV. LEV was synthesized by using a modification of the Merrifield solid phase peptide synthesis technique in which amino acids are coupled sequentially to a solid resin to yield the peptide with the sequence Leu-Glu-Val.The LEV containing peptide was then cleaved from the resin.The gas phase fragmentation property of LEV was examined using a triple quadrupole mass spectrometer equipped with an electrospray ionization (ESI) source. Upon MS/MS analyzation, the protonated LEV produced three characteristic fragment ions shown as three peaks in the mass spectrum.The spectra data agree with the theoretical predictions for the dissociation of a peptide with the sequence LEV. The corresponding peaks were also observed in the mass spectrum for protonated LDV. The protonated LEV and LDV have similar gas phase fragmentation properties.
Location
Pacific Geosciences Center
Start Date
30-4-2005 1:00 PM
End Date
30-4-2005 3:00 PM
Synthesis and Evaluation of the Peptide LEV
Pacific Geosciences Center
The tri-peptide Leu-Glu-Val (LEV) has a structure similar to that of Leu-Asp-Val (LDV). Studies indicate that peptides containing LDV have potential to serve as a target specific drug delivery carriers. LEV was synthesized and its gas phase fragmentation property was evaluated as compared to LDV. LEV was synthesized by using a modification of the Merrifield solid phase peptide synthesis technique in which amino acids are coupled sequentially to a solid resin to yield the peptide with the sequence Leu-Glu-Val.The LEV containing peptide was then cleaved from the resin.The gas phase fragmentation property of LEV was examined using a triple quadrupole mass spectrometer equipped with an electrospray ionization (ESI) source. Upon MS/MS analyzation, the protonated LEV produced three characteristic fragment ions shown as three peaks in the mass spectrum.The spectra data agree with the theoretical predictions for the dissociation of a peptide with the sequence LEV. The corresponding peaks were also observed in the mass spectrum for protonated LDV. The protonated LEV and LDV have similar gas phase fragmentation properties.