Title

An analysis of the RNA Expression Patterns of two genes predicted to aid in the repair of DNA damage in the Fruit Fly Drosophila melanogaster

Poster Number

12

Format

Poster Presentation

Abstract/Artist Statement

Quantitative Real Time PCR is a method for characterizing gene expression patterns and levels amongst several different samples. In our study, we are interested in the expression patterns of DmXRCC2 and DmRad51D in various developmental stages of Drosophila melanogaster (fruit flies). Based on homology with human proteins, DmXRCC2 and DmRad51D are thought to help in DNA repair in mitotic cells. However, they are also similar in appearance to other fly genes that help form DNA crossover events in meiosis. By looking at the expression pattern of DmXRCC2 and DmRad51D, we can collect data on whether they are acting in meiosis or mitosis, or both. We will use real-time PCR to examine mRNA levels from collections of embryos, larvae and adult males and females to see when these genes are expressed. By performing real-time PCR on the various stages at the same time with careful controls, we can compare the relative quantities of the genes expressed. This allows us to develop a developmental profile of gene expression for DmXRCC2 and DmRad51D.

Location

Pacific Geosciences Center

Start Date

24-4-2004 9:00 AM

End Date

24-4-2004 5:00 PM

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Apr 24th, 9:00 AM Apr 24th, 5:00 PM

An analysis of the RNA Expression Patterns of two genes predicted to aid in the repair of DNA damage in the Fruit Fly Drosophila melanogaster

Pacific Geosciences Center

Quantitative Real Time PCR is a method for characterizing gene expression patterns and levels amongst several different samples. In our study, we are interested in the expression patterns of DmXRCC2 and DmRad51D in various developmental stages of Drosophila melanogaster (fruit flies). Based on homology with human proteins, DmXRCC2 and DmRad51D are thought to help in DNA repair in mitotic cells. However, they are also similar in appearance to other fly genes that help form DNA crossover events in meiosis. By looking at the expression pattern of DmXRCC2 and DmRad51D, we can collect data on whether they are acting in meiosis or mitosis, or both. We will use real-time PCR to examine mRNA levels from collections of embryos, larvae and adult males and females to see when these genes are expressed. By performing real-time PCR on the various stages at the same time with careful controls, we can compare the relative quantities of the genes expressed. This allows us to develop a developmental profile of gene expression for DmXRCC2 and DmRad51D.