An analysis of the RNA Expression Patterns of two genes predicted to aid in the repair of DNA damage in the Fruit Fly Drosophila melanogaster
Poster Number
12
Format
Poster Presentation
Abstract/Artist Statement
Quantitative Real Time PCR is a method for characterizing gene expression patterns and levels amongst several different samples. In our study, we are interested in the expression patterns of DmXRCC2 and DmRad51D in various developmental stages of Drosophila melanogaster (fruit flies). Based on homology with human proteins, DmXRCC2 and DmRad51D are thought to help in DNA repair in mitotic cells. However, they are also similar in appearance to other fly genes that help form DNA crossover events in meiosis. By looking at the expression pattern of DmXRCC2 and DmRad51D, we can collect data on whether they are acting in meiosis or mitosis, or both. We will use real-time PCR to examine mRNA levels from collections of embryos, larvae and adult males and females to see when these genes are expressed. By performing real-time PCR on the various stages at the same time with careful controls, we can compare the relative quantities of the genes expressed. This allows us to develop a developmental profile of gene expression for DmXRCC2 and DmRad51D.
Location
Pacific Geosciences Center
Start Date
24-4-2004 9:00 AM
End Date
24-4-2004 5:00 PM
An analysis of the RNA Expression Patterns of two genes predicted to aid in the repair of DNA damage in the Fruit Fly Drosophila melanogaster
Pacific Geosciences Center
Quantitative Real Time PCR is a method for characterizing gene expression patterns and levels amongst several different samples. In our study, we are interested in the expression patterns of DmXRCC2 and DmRad51D in various developmental stages of Drosophila melanogaster (fruit flies). Based on homology with human proteins, DmXRCC2 and DmRad51D are thought to help in DNA repair in mitotic cells. However, they are also similar in appearance to other fly genes that help form DNA crossover events in meiosis. By looking at the expression pattern of DmXRCC2 and DmRad51D, we can collect data on whether they are acting in meiosis or mitosis, or both. We will use real-time PCR to examine mRNA levels from collections of embryos, larvae and adult males and females to see when these genes are expressed. By performing real-time PCR on the various stages at the same time with careful controls, we can compare the relative quantities of the genes expressed. This allows us to develop a developmental profile of gene expression for DmXRCC2 and DmRad51D.