Protein Purification in Fkbp12

Poster Number

10

Format

Poster Presentation

Abstract/Artist Statement

The objective of our research was to purify human Fkbp12 protein expressed in the yeast Pichia pastoris. Purification methods included nickel, cobalt and primary immunoprecipitation where the antigen Fkbp12-anti-myc will bind to the antibody and eluted through centrifugation. The results were detected by SDS-page and Western blot. The results will be display on our poster.

Location

Pacific Geosciences Center

Start Date

24-4-2004 9:00 AM

End Date

24-4-2004 5:00 PM

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Apr 24th, 9:00 AM Apr 24th, 5:00 PM

Protein Purification in Fkbp12

Pacific Geosciences Center

The objective of our research was to purify human Fkbp12 protein expressed in the yeast Pichia pastoris. Purification methods included nickel, cobalt and primary immunoprecipitation where the antigen Fkbp12-anti-myc will bind to the antibody and eluted through centrifugation. The results were detected by SDS-page and Western blot. The results will be display on our poster.