A quick & easy transformation method for Pichia pastoris.
Poster Number
8
Format
Poster Presentation
Abstract/Artist Statement
There are 2 traditional methods to transform DNA into yeast cells. One method is using chemical treatment (sold as Easy Comp kit by Invitrogen Corp. Carlsbad, CA). The second method is electroporation, which uses electricity to make the cell walls, of yeast, more permeable. Each method has its benefits and drawbacks. Easy Comp procedure is quite long and requires many steps. However, preparation steps to make the yeast cells are quite simple and require little time. The transformation yield of Easy Comp is quite low compared electroporation. Electroporation procedures are quite short. The drawback though, is that the preparation steps are lengthy. Overall, electroporation results in a high yield. The objectives of these experiments are to combine the preparation procedures of Easy Comp with the transformation procedures of electroporation. In theory, this method should be the most time efficient process and with transformation efficiency similar of that of electroporation.
Location
Pacific Geosciences Center
Start Date
26-4-2003 9:00 AM
End Date
26-4-2003 5:00 PM
A quick & easy transformation method for Pichia pastoris.
Pacific Geosciences Center
There are 2 traditional methods to transform DNA into yeast cells. One method is using chemical treatment (sold as Easy Comp kit by Invitrogen Corp. Carlsbad, CA). The second method is electroporation, which uses electricity to make the cell walls, of yeast, more permeable. Each method has its benefits and drawbacks. Easy Comp procedure is quite long and requires many steps. However, preparation steps to make the yeast cells are quite simple and require little time. The transformation yield of Easy Comp is quite low compared electroporation. Electroporation procedures are quite short. The drawback though, is that the preparation steps are lengthy. Overall, electroporation results in a high yield. The objectives of these experiments are to combine the preparation procedures of Easy Comp with the transformation procedures of electroporation. In theory, this method should be the most time efficient process and with transformation efficiency similar of that of electroporation.