Construction and screening of new EGFP-PTSI1 vectors in pichia pastoris
Poster Number
24
Format
Poster Presentation
Abstract/Artist Statement
EGFP-PTS1 is a fusion of the gene for enhanced green fluorescent protein and a peroxisome targeting sequence. Originally this complex was inserted into a vector with zeocin as a selectable marker. Zeocin is an expensive antibiotic to work with. Amino acids such as histidine and adenine are less expensive to use. In order to generate a similar vector based on amino acid auxotrophy, the EGFP-PTS1 gene will be removed from the zeocin vector and inserted into vectors selecting for either adenine or histidine. Through different screening techniques I hope to determine which new complexes are accurate and functioning.
Location
Pacific Geosciences Center
Start Date
20-4-2002 9:00 AM
End Date
20-4-2002 5:00 PM
Construction and screening of new EGFP-PTSI1 vectors in pichia pastoris
Pacific Geosciences Center
EGFP-PTS1 is a fusion of the gene for enhanced green fluorescent protein and a peroxisome targeting sequence. Originally this complex was inserted into a vector with zeocin as a selectable marker. Zeocin is an expensive antibiotic to work with. Amino acids such as histidine and adenine are less expensive to use. In order to generate a similar vector based on amino acid auxotrophy, the EGFP-PTS1 gene will be removed from the zeocin vector and inserted into vectors selecting for either adenine or histidine. Through different screening techniques I hope to determine which new complexes are accurate and functioning.