Title

The expression of C. Elegans SPE-9 receptor in P. Pastoris

Poster Number

21

Format

Poster Presentation

Abstract/Artist Statement

The use of the Pichia pastoris yeast allows for efficient heterologous protein secretion. The SPE-9 extracellular domain is cloned into two Pichia expression vectors, pPICZB and pPICZαB. The differences in the two vectors are that pPICZB expresses heterologous proteins intracellularly, whereas pPICZαB contains an α-mating factor signal sequence, which results in extracellular protein secretion. The SPE-9 gene encodes for a sperm transmembrane protein required for fertilization in the nematode worm Caenohabditis elegans. SPE-9 functions as a sperm ligand during gamete interactions. Production of SPE-9 protein will allow us to further understand the functional roles of various genes required for fertilization, reproduction, and development.

Location

Pacific Geosciences Center

Start Date

20-4-2002 9:00 AM

End Date

20-4-2002 5:00 PM

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Apr 20th, 9:00 AM Apr 20th, 5:00 PM

The expression of C. Elegans SPE-9 receptor in P. Pastoris

Pacific Geosciences Center

The use of the Pichia pastoris yeast allows for efficient heterologous protein secretion. The SPE-9 extracellular domain is cloned into two Pichia expression vectors, pPICZB and pPICZαB. The differences in the two vectors are that pPICZB expresses heterologous proteins intracellularly, whereas pPICZαB contains an α-mating factor signal sequence, which results in extracellular protein secretion. The SPE-9 gene encodes for a sperm transmembrane protein required for fertilization in the nematode worm Caenohabditis elegans. SPE-9 functions as a sperm ligand during gamete interactions. Production of SPE-9 protein will allow us to further understand the functional roles of various genes required for fertilization, reproduction, and development.