The expression of C. Elegans SPE-9 receptor in P. Pastoris
Poster Number
21
Format
Poster Presentation
Abstract/Artist Statement
The use of the Pichia pastoris yeast allows for efficient heterologous protein secretion. The SPE-9 extracellular domain is cloned into two Pichia expression vectors, pPICZB and pPICZαB. The differences in the two vectors are that pPICZB expresses heterologous proteins intracellularly, whereas pPICZαB contains an α-mating factor signal sequence, which results in extracellular protein secretion. The SPE-9 gene encodes for a sperm transmembrane protein required for fertilization in the nematode worm Caenohabditis elegans. SPE-9 functions as a sperm ligand during gamete interactions. Production of SPE-9 protein will allow us to further understand the functional roles of various genes required for fertilization, reproduction, and development.
Location
Pacific Geosciences Center
Start Date
20-4-2002 9:00 AM
End Date
20-4-2002 5:00 PM
The expression of C. Elegans SPE-9 receptor in P. Pastoris
Pacific Geosciences Center
The use of the Pichia pastoris yeast allows for efficient heterologous protein secretion. The SPE-9 extracellular domain is cloned into two Pichia expression vectors, pPICZB and pPICZαB. The differences in the two vectors are that pPICZB expresses heterologous proteins intracellularly, whereas pPICZαB contains an α-mating factor signal sequence, which results in extracellular protein secretion. The SPE-9 gene encodes for a sperm transmembrane protein required for fertilization in the nematode worm Caenohabditis elegans. SPE-9 functions as a sperm ligand during gamete interactions. Production of SPE-9 protein will allow us to further understand the functional roles of various genes required for fertilization, reproduction, and development.