Identification of novel ABF-1 interacting proteins using the yeast 2-hybrid system.
Poster Number
6
Format
Poster Presentation
Abstract/Artist Statement
Basic Helix-loop-Helix (bHLH) proteins are involved in cellular differentiation and proliferation. Recently, a bHLH tissue-restricted protein, known as activated B-cell factor (ABF-1) has been identified. To date, ABF-1 represents the only B -cellrestricted protein identified. ABF-1 is expressed.in B-cells treated with mitogen, suggesting a role in B-cell proliferation. In addition elevated levels of ABF-1 are displayed in B-cells infected with Epstein-Barr virus (EBV), an agent known to immortalize B-cells. In order to identify novel protein interactions with ABF-1 a eDNA library, isolated from EBV transformed B-cells, was screened using the yeast 2-hybrid system. The yeast 2-hybrid system is a technique commonly employed to identify protein interactions in vivo. The results obtained from this screening procedure will ];)e discussed.
Location
DeRosa University Center
Start Date
1-5-2001 9:00 AM
End Date
1-5-2001 5:00 PM
Identification of novel ABF-1 interacting proteins using the yeast 2-hybrid system.
DeRosa University Center
Basic Helix-loop-Helix (bHLH) proteins are involved in cellular differentiation and proliferation. Recently, a bHLH tissue-restricted protein, known as activated B-cell factor (ABF-1) has been identified. To date, ABF-1 represents the only B -cellrestricted protein identified. ABF-1 is expressed.in B-cells treated with mitogen, suggesting a role in B-cell proliferation. In addition elevated levels of ABF-1 are displayed in B-cells infected with Epstein-Barr virus (EBV), an agent known to immortalize B-cells. In order to identify novel protein interactions with ABF-1 a eDNA library, isolated from EBV transformed B-cells, was screened using the yeast 2-hybrid system. The yeast 2-hybrid system is a technique commonly employed to identify protein interactions in vivo. The results obtained from this screening procedure will ];)e discussed.