The role of notch in the expression of ABF-1.

Poster Number

5

Format

Poster Presentation

Abstract/Artist Statement

Activated B-cell factor (ABF-1) is a basic helix-loop-helix transcription factor that plays a role in the proliferation and differentiation ofB-cells and muscle cells. The regulation of expression of the ABF-1 gene is not clearly defined, however B-cells infected with the Epstein Barr Virus show increased levels of ABF -1. The activation of a ubiquitous surface receptor, known as Notch, has been shown to regulate expression of genes analogously to Epstein Barr Virus infected cells. The affect of activated Notch on the 5' flanking region of the ABF-1 gene was investigated. Notch activated the 5' flanking region of the ABF -1 gene in transfected HeLa cells, and the region of activation was narrowed to a 1kb segment. The significance of these results will be discussed.

Location

DeRosa University Center

Start Date

1-5-2001 9:00 AM

End Date

1-5-2001 5:00 PM

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May 1st, 9:00 AM May 1st, 5:00 PM

The role of notch in the expression of ABF-1.

DeRosa University Center

Activated B-cell factor (ABF-1) is a basic helix-loop-helix transcription factor that plays a role in the proliferation and differentiation ofB-cells and muscle cells. The regulation of expression of the ABF-1 gene is not clearly defined, however B-cells infected with the Epstein Barr Virus show increased levels of ABF -1. The activation of a ubiquitous surface receptor, known as Notch, has been shown to regulate expression of genes analogously to Epstein Barr Virus infected cells. The affect of activated Notch on the 5' flanking region of the ABF-1 gene was investigated. Notch activated the 5' flanking region of the ABF -1 gene in transfected HeLa cells, and the region of activation was narrowed to a 1kb segment. The significance of these results will be discussed.