Chemical identification and evaluation of pheromones from the axial breeding glands of hymenochirus sp. African dwarf frogs.

Poster Number

12

Format

Poster Presentation

Abstract/Artist Statement

Sexually dimorphic skin glands in Hymenochirus sp. anurans have been shown to secrete a sexual attractant to attract conspecific females (Pearl eta!., 2000). Here these glands were studied for their chemical properties in order to determine the nature of the attractant. The first phase of the project involves the removal of the glands from the frogs and chemical analysis of the gland homogenate by means of High Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS). The second phase of the experiment was concerned with using HPLC to examine secretions from the male frogs into deionized water. The HPLC technique employed proved useful in chromatography of the gland and in relating the gland to the secretion product. MS results were show some dissolved substances, but are not yet conclusive.

Location

DeRosa University Center

Start Date

1-5-2001 9:00 AM

End Date

1-5-2001 5:00 PM

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May 1st, 9:00 AM May 1st, 5:00 PM

Chemical identification and evaluation of pheromones from the axial breeding glands of hymenochirus sp. African dwarf frogs.

DeRosa University Center

Sexually dimorphic skin glands in Hymenochirus sp. anurans have been shown to secrete a sexual attractant to attract conspecific females (Pearl eta!., 2000). Here these glands were studied for their chemical properties in order to determine the nature of the attractant. The first phase of the project involves the removal of the glands from the frogs and chemical analysis of the gland homogenate by means of High Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS). The second phase of the experiment was concerned with using HPLC to examine secretions from the male frogs into deionized water. The HPLC technique employed proved useful in chromatography of the gland and in relating the gland to the secretion product. MS results were show some dissolved substances, but are not yet conclusive.