Measurement of Tissue Oxidation-Reduction State with Carbon-13 Nuclear Magnetic Resonance Spectroscopy

ORCiD

0000-0001-9010-5970

Document Type

Article

Publication Title

Cancer Research

ISSN

0008-5472

Volume

49

Issue

8

First Page

1937

Last Page

1940

Publication Date

4-1-1989

Abstract

The oxidation state of tissues influences their response to cancer therapy. We have devised a novel approach to the measurement of thiol redox which is based on the relative nuclear magnetic resonance signal intensity from carbon-13 adjacent to sulfur in metabolites of the redox-sensitive phosphorothioate drug, S-2-(3-methylaminopropylamino)ethylphosphorothioic acid (WR3689). Incubation of WR3689 metabolites under oxidizing conditions results in quantifiable changes in the 13C nuclear magnetic resonance spectrum stoichiometrically related to the degree of oxidation in mouse liver homogenate in vitro. Drug oxidation is competitive with the oxidation of tissue-derived thiol groups under these conditions. Noninvasive measurement of redox state may assist in designing more effective strategies for altering normal and malignant tissue response to cancer therapy.

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