Presentation Category
Research
Introduction/Context/Diagnosis
Nonsyndromic cleft lip with or without cleft palate (NSCLP) occurs approximately in 1 of 700 live births. It has a serious impact on a patient during the whole life. It was shown that it can be prevented by supplementation of folic acid. However, much more needs to be known about etiopathogenetic factors and molecular mechanisms leading to NSCLP in order to personalize its prevention. Basic science information on etiology and pathogenesis of NSCLP was obtained from animal experiments. Clinical studies and trials showed efficacy of prevention by folic acid supplementation. A cell culture model is needed for studies on cellular and molecular mechanisms leading to development of NSCLP and underlying its prevention. Our goal is to compare behavior of HDPSC isolated from patients with or without NSCLP.
Methods/Treatment Plan
NSCLP develops due to compromise of neural crest cells in embryo. Human dental pulp stem cells (HDPSC) are descendants of neural crest cells. We isolate HDPSC from extracted teeth of patients with or without NSCLP in our school (IRB #2021-80).
Results/Outcome
A method of HDPSC isolation and cultivation in a monolayer culture is described. Our previous studies showed that endoplasmic reticulum stress leading to compromise of HDPSC can be induced by a transient severe hypoxia. Folic acid increased survival of HDPSC after a hypoxic episode.
Significance/Conclusions
A future study will compare properties of HDPSC isolated from extracted teeth of patients with or without NSCLP and their sensitivity to folate supplementation.
Format
Event
Human dental pulp stem cell culture model for studies on etiopathogenesis of nonsyndromic cleft lip with or without cleft palate anomaly
Nonsyndromic cleft lip with or without cleft palate (NSCLP) occurs approximately in 1 of 700 live births. It has a serious impact on a patient during the whole life. It was shown that it can be prevented by supplementation of folic acid. However, much more needs to be known about etiopathogenetic factors and molecular mechanisms leading to NSCLP in order to personalize its prevention. Basic science information on etiology and pathogenesis of NSCLP was obtained from animal experiments. Clinical studies and trials showed efficacy of prevention by folic acid supplementation. A cell culture model is needed for studies on cellular and molecular mechanisms leading to development of NSCLP and underlying its prevention. Our goal is to compare behavior of HDPSC isolated from patients with or without NSCLP.
Comments/Acknowledgements
This study was partially supported by DRES03-115 Research Award from the Research Committee of the Dugoni School of Dentistry, UOP.