Mixed and Purified SCAPs: Similar Growth, Phenotypic and Differentiation Characteristics

Authors

Julian G. Leprince, School of Dentistry and Stomatology, Université catholique de LouvainFollow
Benjamin David Zeitlin, University of the PacficFollow
Miroslav Tolar, University of the PacificFollow
Anibal Diogenes, University of Texas Health Science Center at San AntonioFollow
Ove A. Peters, University of the PacificFollow

ORCiD

Dr. Benjamin D. Zeitlin: 0000-0003-0110-0188

Department

Biomedical Sciences

Document Type

Conference Presentation

Conference Title

General Session & Exhibition of the IADR

Organization

International Association for Dental Research

Location

San Francisco, CA

Date of Presentation

4-1-2012

Abstract

Objective: To compare the phenotype, the growth curve and the osteo- and chondrogenic differentiation potential of mixed vs purified stem cells populations from the apical papilla (SCAPs); gingival fibroblasts (GFs) were used as control. Method: Four cell populations were considered: 1)mixed SCAPs (M-SCAPs); 2&3)SCAPs purified by immunomagnetic separation based either on Stro-1 (P-SCAPs1), or on a combination of CD73, CD90 and CD105 (P-SCAPs2); 4)gingival fibroblasts (GFs). Each population was subcultured, and their growth curve determined. Their phenotype was characterized by flow cytometry using antibodies against CD29, CD31, CD34, CD90, CD105 and Stro-1. The cells were then cultured in osteo- and chondrogenic media during 25 days. Osteogenic and chondrogenic differentiation were evaluated respectively by alizarin red S and alcian blue staining. Result: All four cell populations had similar growth curves, were homogeneously positive to CD29, CD90 and CD105 (percentage of positive cells >97% in all cases but P-SCAPs1 for CD90 – 91.5%), and negative to CD31, CD34 and Stro-1 (<3% in all cases but GFs for CD34 – 7.5%). All cell populations were positive for alizarin red S, and all but GFs were positive for alcian blue. Conclusion: The present results question the specificity of certain markers considered to identify dental mesenchymal stem cells. The expression of Stro-1 was notably lost in P-SCAPs1, which however conserved their multi-differentiation potential. The rationale of sorting cells based on these markers is therefore called into question vs using a mixed population. Finally, the results suggest that GFs either contain a certain proportion of osteogenic progenitor cells, or that they intrinsically possess an osteogenic differentiation potential.

Recommended Citation

Leprince, J. G., Zeitlin, B. D., Tolar, M., Diogenes, A., & Peters, O. A. (2012). Mixed and Purified SCAPs: Similar Growth, Phenotypic and Differentiation Characteristics. Paper presented at General Session & Exhibition of the IADR in San Francisco, CA.
https://scholarlycommons.pacific.edu/dugoni-facpres/84