Neurotrophins BDNF and NT4/5 accelerate dental pulp stem cell migration

Department

Biomedical Sciences

Document Type

Article

Publication Title

Biomedical Journal

ISSN

2319-4170

Volume

44

Issue

3

DOI

10.1016/j.bj.2020.03.010

First Page

363

Last Page

368

Publication Date

6-1-2021

Abstract

Neurotrophic factors play important roles in neuron survival, growth and differentiation. In the present research, the expression of multiple neurotrophins and their effects on cell migration were studied in the dental pulp stem cells (DPSCs). Human DPSCs from five patients were cultured. Expression of neurotrophins and their receptors were evaluated by PCR, immunofluorescent staining and ELISA. Scratch assay was performed in the presence or absence of neurotrophic factors. Level of phosphorylated-ERK was evaluated with Western blotting. Neurotrophins were expressed at various levels in the DPSCs. Treatment of 100 ng/ml BDNF or NT4/5 accelerated wound healing in scratch assay and elevated the expression of phosphorylated–ERK. The work indicated that neurotrophins promoted human DPSCs migration in vitro.

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