Department
Biomedical Sciences
Document Type
Article
Publication Title
Frontiers in Immunology
ISSN
1664-3224
DOI
10.3389/fimmu.2021.714090
First Page
3329
Publication Date
9-1-2021
Abstract
Although most causes of death and morbidity in premature infants are related to immune maladaptation, the premature immune system remains poorly understood. We provide a comprehensive single-cell depiction of the neonatal immune system at birth across the spectrum of viable gestational age (GA), ranging from 25 weeks to term. A mass cytometry immunoassay interrogated all major immune cell subsets, including signaling activity and responsiveness to stimulation. An elastic net model described the relationship between GA and immunome (R=0.85, p=8.75e-14), and unsupervised clustering highlighted previously unrecognized GA-dependent immune dynamics, including decreasing basal MAP-kinase/NFkB signaling in antigen presenting cells; increasing responsiveness of cytotoxic lymphocytes to interferon-a; and decreasing frequency of regulatory and invariant T cells, including NKT cells and MAIT cells. Knowledge gained from the analysis of the neonatal immune landscape across GA provides a mechanistic framework to understand the unique susceptibility of preterm infants to both hyper-inflammatory diseases and infections.
Recommended Citation
Peterson, L. S.,
Hedou, J.,
Ganio, E.,
Stelzer, I. A.,
Feyaerts, D.,
Harbert, E.,
Adusumelli, Y.,
Ando, K.,
Tsai, E. S.,
Tsai, A. S.,
Han, X.,
Ringle, M.,
Houghteling, P.,
Reiss, J.,
Lewis, D.,
Winn, V. D.,
Angst, M. S.,
Aghaeepour, N.,
Stevenson, D. K.,
&
Gaudilliere, B.
(2021).
Single-cell Analysis of the Neonatal Immune System Across the Gestational Age Continuum.
Frontiers in Immunology, , 3329.
DOI: 10.3389/fimmu.2021.714090
https://scholarlycommons.pacific.edu/dugoni-facarticles/731
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
