ORCiD
Cassio Almeida-da-Silva: 0000-0001-9173-7208
Department
Biomedical Sciences
Document Type
Article
Publication Title
BioMed Research International
ISSN
2314-6133
Volume
2013
DOI
10.1155/2013/474132
First Page
1
Last Page
11
Publication Date
9-22-2013
Abstract
Allergic airway inflammation is attenuated by oral tolerization (oral exposure to allergen, followed by conventional sensitization and challenge with homologous antigen), which decreases airway allergen challenge-induced eosinophilic infiltration of the lungs and bone marrow eosinophilia. We examined its effects on bone marrow eosinophil and neutrophil production. Mice of wild type (BP-2, BALB/c, and C57BL/6) and mutant strains (lacking iNOS or CD95L) were given ovalbumin (OVA) or water (vehicle) orally and subsequently sensitized and challenged with OVA (OVA/OVA/OVA and H2O/OVA/OVA groups, resp.). Anti-OVA IgG and IgE, bone marrow eosinophil and neutrophil numbers, and eosinophil and neutrophil production ex vivo were evaluated. T lymphocytes from OVA/OVA/OVA or control H2O/OVA/OVA donors were transferred into naïve syngeneic recipients, which were subsequently sensitized/challenged with OVA. Alternatively, T lymphocytes were cocultured with bone marrow eosinophil precursors from histocompatible sensitized/challenged mice. OVA/OVA/OVA mice of the BP-2 and BALB/c strains showed, relative to H2O/OVA/OVA controls, significantly decreased bone marrow eosinophil counts and ex vivo eosinopoiesis/neutropoiesis. Full effectiveness in vivo required sequential oral/subcutaneous/intranasal exposures to the same allergen. Transfer of splenic T lymphocytes from OVA/OVA/OVA donors to naive recipients prevented bone marrow eosinophilia and eosinopoiesis in response to recipient sensitization/challenge and supressed eosinopoiesis upon coculture with syngeneic bone marrow precursors from sensitized/challenged donors.
Recommended Citation
Xavier-Elsas, P.,
Almeida-Da-Silva, C. L.,
Pinto, L.,
Queto, T.,
Marques. B.,
Aramha, M. G.,
De Luca, B.,
Masid-de-Brito, D.,
Luz, R. A.,
Lopes, R. S.,
Ferreira, R.,
&
Gaspar-Elsas, M. I.
(2013).
Modulation of the Effects of Lung Immune Response on Bone Marrow by Oral Antigen Exposure.
BioMed Research International, 2013, 1–11.
DOI: 10.1155/2013/474132
https://scholarlycommons.pacific.edu/dugoni-facarticles/710
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
