ORCiD
Nejat Düzgüneş: 0000-0001-6159-1391
Department
Biomedical Sciences
Document Type
Article
Publication Title
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424
Volume
79
Issue
15
DOI
10.1073/pnas.79.15.4642
First Page
4642
Last Page
4644
Publication Date
1-1-1982
Abstract
The effect of synexin (an adrenal medullary protein) on the kinetics of Ca2+- and Mg2+-mediated membrane fusion was examined. Membrane fusion was studied by monitoring intermixing of the aqueous contents of phospholipid vesicles. Synexin facilitated Ca2+-mediated, but not Mg2+-mediated, fusion of phosphatidate/phosphatidylethanolamine (1:3) and phosphatidate/phosphatidylserine/phosphatidylethanolamine/cholesterol (1:2:3:2) vesicles. The threshold concentration of Ca2+ for fusion was decreased to approximately equal to 10 microM in the presence of synexin at 6 micrograms/ml and 1.5 mM Mg2+ in vesicle suspensions containing 50 microM lipid. This effect of synexin was drastically inhibited by including 25% phosphatidylcholine (mol/mol) in the vesicle membrane. It is proposed that the Ca2+-dependent lipid-specific enhancement of membrane fusion by synexin contributes to an increase in the sensitivity of specific intracellular membranes to Ca2+ with respect to fusion.
Recommended Citation
Hong, K.,
Düzgüneş, N.,
Ekerdt, R.,
&
Papahadjopoulos, D.
(1982).
Synexin facilitates fusion of specific phospholipid membranes at divalent cation concentrations found intracellularly..
Proceedings of the National Academy of Sciences of the United States of America, 79(15), 4642–4644.
DOI: 10.1073/pnas.79.15.4642
https://scholarlycommons.pacific.edu/dugoni-facarticles/685
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