ORCiD
Nejat Düzgüneş: 0000-0001-6159-1391
Department
Biomedical Sciences
Document Type
Article
Publication Title
Journal of Leukocyte Biology
ISSN
0741-5400
Volume
65
Issue
2
DOI
10.1002/jlb.65.2.270
First Page
270
Last Page
279
Publication Date
1-1-1999
Abstract
The crucial function of macrophages in a variety of biological processes and pathologies render these cells important targets for gene therapeutic interventions. Commonly used synthetic gene delivery vectors have not been successful in transfecting these non-dividing cells. A combination strategy involving cationic liposomes to condense and carry DNA, transferrin to facilitate cellular uptake, and the pH-sensitive peptide GALA to promote endosome destabilization, resulted in significant expression of a luciferase gene. Transfection of macrophages was dependent on the degree of differentiation of the cells. The quaternary complexes of cationic liposomes, DNA, transferrin, and GALA exhibited a net negative charge, which may obviate a limitation of cationic synthetic vectors in vivo. The lack of cytotoxicity and the expected back of immunogenicity of these complexes may render them useful for gene delivery to macrophages in vivo.
Recommended Citation
Simões, S.,
Slepushkin, V.,
Pretzer, E.,
Dazin, P.,
Gaspar, R.,
Pedroso De Lima, M. C.,
&
Düzgüneş, N.
(1999).
Transfection of human macrophages by lipoplexes via the combined use of transferrin and pH-sensitive peptides.
Journal of Leukocyte Biology, 65(2), 270–279.
DOI: 10.1002/jlb.65.2.270
https://scholarlycommons.pacific.edu/dugoni-facarticles/664
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
