ORCiD
David M. Ojcius: 0000-0003-1461-4495
Department
Biomedical Sciences
Document Type
Article
Publication Title
PLoS One
ISSN
1932-6203
Volume
6
Issue
4
DOI
10.1371/journal.pone.0018308
First Page
1
Last Page
11
Publication Date
4-12-2011
Abstract
Osajin is a prenylated isoflavone showing antitumor activity in different tumor cell lines. The underlying mechanism of osajin-induced cancer cell death is not clearly understood. In the present study, the mechanisms of osajin-induced cell death of human nasopharyngeal carcinoma (NPC) cells were explored. Osajin was found to significantly induce apoptosis of NPC cells in a dose- and time-dependent manner. Multiple molecular effects were observed during osajin treatment including a significant loss of mitochondrial transmembrane potential, release of cytochrome c into the cytosol, enhanced expression of Fas ligand (FasL), suppression of glucose-regulated protein 78 kDa (GRP78), and activation of caspases-9, -8, -4 and -3. In addition, up-regulation of proapoptotic Bax protein and down-regulation of antiapoptotic Bcl-2 protein were also observed. Taken together, osajin induces apoptosis in human NPC cells through multiple apoptotic pathways, including the extrinsic death receptor pathway, and intrinsic pathways relying on mitochondria and endoplasmic reticulum stress. Thus, osajin could be developed as a new effective and chemopreventive compound for human NPC.
Recommended Citation
Huang, T.,
Liu, F.,
Wei, C.,
Lu, C.,
Chen, C.,
Lin, H.,
Ojcius, D. M.,
&
Lai, H.
(2011).
Activation of multiple apoptotic pathways in human nasopharyngeal carcinoma cells by the prenylated isoflavone, osajin.
PLoS One, 6(4), 1–11.
DOI: 10.1371/journal.pone.0018308
https://scholarlycommons.pacific.edu/dugoni-facarticles/65
Included in
Biochemistry Commons, Immunity Commons, Immunology of Infectious Disease Commons, Medical Immunology Commons
Comments
Article e18308