ORCiD
Nejat Düzgüneş: 0000-0001-6159-1391
Department
Biomedical Sciences
Document Type
Article
Publication Title
FEBS Letters
ISSN
0014-5793
Volume
179
Issue
1
DOI
10.1016/0014-5793(85)80210-6
First Page
148
Last Page
154
Publication Date
1-1-1985
Abstract
Negatively charged liposomes are endocytosed by the coated vesicle system and accumulate in acidic intracellular vesicles. Liposomes that become unstable at acidic pH improve cytoplasmic delivery of membraneimpermeant macromolecules such as calcein (CAL) and FITC dextran (18 or 40 kDa). Oleic acid (OA): phosphatidylethanolamine (PE) (3:7 mole ratio) liposomes become permeable to CAL at pH < 7.0. Control liposomes of phosphatidylserine : PE or OA : phosphatidylcholine are stable at pH 4-8. OA:PE liposomes promote cytoplasmic delivery of encapsulated CAL to CV-1 cells, as evidenced by the emergence of diffuse, cytoplasmic CAL fluorescence. Delivery requires metabolic energy and is partially inhibited by chloroquine or monensin, which raise the pH of intracellular vesicles. © 1985.
Recommended Citation
Straubinger, R. M.,
Düzgüneş, N.,
&
Papahadjopoulos, D.
(1985).
pH-sensitive liposomes mediate cytoplasmic delivery of encapsulated macromolecules.
FEBS Letters, 179(1), 148–154.
DOI: 10.1016/0014-5793(85)80210-6
https://scholarlycommons.pacific.edu/dugoni-facarticles/625
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
