"Unusual sponge phospholipids and their analogs. Synthesis and interact" by Eser Ayanoglu, Huiting Li et al.
 

Unusual sponge phospholipids and their analogs. Synthesis and interactions with conventional phospholipids and cholesterol in model membranes

ORCiD

Nejat Düzgüneş: 0000-0001-6159-1391

Department

Biomedical Sciences

Document Type

Article

Publication Title

Chemistry and Physics of Lipids

ISSN

0009-3084

Volume

47

Issue

3

DOI

10.1016/0009-3084(88)90010-2

First Page

165

Last Page

175

Publication Date

1-1-1988

Abstract

As reported earlier, the marine sponge phospholipids 1,2-di-5,9-hexacosadienoyl-sn-glycero-3-phosphocholine (PC: (26 : 2, 26 : 2)) and its phosphoethanolamine counterpart exclude cholesterol to a great extent from their liposomal bilayers. To understand the reason for this exclusion, shorter acyl chain analogs of these phospholipids were synthesized. Liposomes composed of PC (18 : 2, 18 : 2), PC (22 : 2, 22 : 2) or PC (24 : 2, 24 : 2), with the unique Δ5,9 unsaturation typical of sponge phospholipids, incorporated cholesterol Δ5,9-PE (24 : 2, 24 : 2) which also incorporated cholesterol, had a phase transition temperature identical to its PC counterpart. Differential scanning calorimetry indicated that Δ5,9-PC (26 : 2, 26 : 2) could incorporate 1-stearoyl-2-oleoyl-sn-glycerophosphocholine PC (18 : 0, 18 : 1) into its bilayers at various ratios (20, 40, 60, 80 mol%), but part of PC (18 : 0, 18: 1) displayed monotectic behavior. When 20 mol cholesterol was added to thesemixtures, the sterol interacted preferentially with the PC (18 : 0, 18 : 1) but was also incorporated in all ratios into the mixture of phospholipids and created one major transition endotherm. © 1988.

Share

COinS