ORCiD
Nejat Düzgüneş: 0000-0001-6159-1391
Department
Biomedical Sciences
Document Type
Article
Publication Title
Biophysical Journal
ISSN
0006-3495
Volume
85
Issue
2
DOI
10.1016/S0006-3495(03)74558-5
First Page
1223
Last Page
1232
Publication Date
8-1-2003
Abstract
A minicapillary viscometer utilizing <0.5 ml of sample at a volume fraction of <0.1% is described. The calculated a/b of DPPC/DPPG multilamellar liposome was 1.14 as prolate ellipsoids and a/b of dioleoylpropyltrimethyl ammonium methylsulfate-DNA complex at a charge ratio of 4:1 (+/-) was 3.7 as prolate ellipsoids or 4.9 as oblate ellipsoids. The deviation of shape from perfect sphere is thus expressed quantitatively in more than two significant figures. In these measurement, the necessary amount of DNA is <0.5 mg.
Recommended Citation
Sun, Y.,
Li, X.,
Düzgüneş, N.,
Takaoka, Y.,
Ohi, S.,
&
Hirota, S.
(2003).
The shape parameter of liposomes and DNA-lipid complexes determined by viscometry utilizing small sample volumes.
Biophysical Journal, 85(2), 1223–1232.
DOI: 10.1016/S0006-3495(03)74558-5
https://scholarlycommons.pacific.edu/dugoni-facarticles/593
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
