Role of the CDR1 of the TCR β chain in the binding to a MHC-peptide complex

Authors

Yu-Chun Lone
Maria Bellio
Ada Prochnicka-Chalufour
David M. Ojcius, University of the PacificFollow
Nicolas Boissel
Tom H. M. Ottenhoff
Richard D. Klausner
Jean-Pierre Abastado
Philippe Kourilsky

ORCiD

David M. Ojcius: 0000-0003-1461-4495

Department

Biomedical Sciences

Document Type

Article

Publication Title

International Immunology

ISSN

0953-8178

Volume

6

Issue

10

DOI

10.1093/intimm/6.10.1561

First Page

1561

Last Page

1565

Publication Date

10-1-1994

Abstract

Single alanine substitutions were introduced into the CDR1 region of the β chain of a K^d-restricted TCR. Mutants and wild-type TCR were attached to the ζ chain of the CD3 complex and expressed at the surface of a rat basophil cell line. Transfectants were tested for the binding of purified soluble K^d-peptlde complexes. With this experimental system, accessory molecules are unlikely to play a major role and the contribution of each residue to the interaction can be addressed. Results show that all positions in the CDR1 region are involved in the binding to the K^d-peptide complex but at varying degrees. These effects are discussed in relation to a molecular model of the TCR. Comparison of these results with previous data obtained in a T cell hybridoma system suggests the existence of a threshold in the TCR affinity necessary for mature T cell activation.

Recommended Citation

Lone, Y., Bellio, M., Prochnicka-Chalufour, A., Ojcius, D. M., Boissel, N., Ottenhoff, T. H., Klausner, R. D., Abastado, J., & Kourilsky, P. (1994). Role of the CDR1 of the TCR β chain in the binding to a MHC-peptide complex. International Immunology, 6(10), 1561–1565. DOI: 10.1093/intimm/6.10.1561
https://scholarlycommons.pacific.edu/dugoni-facarticles/49