Leptospira interrogans infection leads to IL-1β and IL-18 secretion from a human macrophage cell line through reactive oxygen species and cathepsin B mediated-NLRP3 inflammasome activation.
ORCiD
David M. Ojcius: 0000-0003-1461-4495
Department
Biomedical Sciences
Document Type
Article
Publication Title
Microbes and Infection
ISSN
1769-714X
Volume
20
Issue
4
DOI
10.1016/j.micinf.2018.01.010
First Page
254
Last Page
260
Publication Date
4-1-2018
Abstract
Leptospirosis is a worldwide zoonosis caused by spirochetes from the genus Leptospira. Although there is a large diversity of clinical signs and symptoms, a severe inflammatory response is common to all leptospirosis patients. The mechanism of IL-1β secretion during Leptospira infection has been previously studied in mouse macrophages. However, the outcome of Leptospira infection is very different in human and murine macrophages, and the mechanisms responsible for IL-1β secretion in human macrophages had not been investigated. This study therefore examines the effects of Leptospira interrogans infection on inflammasome activation and proinflammatory cytokine expression in human macrophages. Increased mRNA and protein expression of NLRP3 was observed by real time RT-PCR and flow cytometry at 1 h after co-cultivation. Enzyme-linked immunosorbent assay (ELISA) determination showed that IL-1β and IL-18 are released in the culture supernatants at 1 h after cultivation. The inhibition assay showed that glybenclamide (a K
Recommended Citation
Li, S.,
Wang, M.,
Ojcius, D. M.,
Zhou, B.,
Hu, W.,
Liu, Y.,
Ma, Q.,
Tang, G.,
Wang, D.,
&
Yan, J.
(2018).
Leptospira interrogans infection leads to IL-1β and IL-18 secretion from a human macrophage cell line through reactive oxygen species and cathepsin B mediated-NLRP3 inflammasome activation..
Microbes and Infection, 20(4), 254–260.
DOI: 10.1016/j.micinf.2018.01.010
https://scholarlycommons.pacific.edu/dugoni-facarticles/437
