ORCiD
Dr. Ove A. Peters: 0000-0001-5222-8718
Department
Endodontics
Document Type
Article
Publication Title
Journal of Applied Oral Science
ISSN
1678-7757
Volume
26
DOI
10.1590/1678-7757-2017-0374
First Page
e20170374
Publication Date
2-8-2018
Abstract
Objectives: To determine the concentration of calcium, iron, manganese and zinc ions after the application of chelator to Enterococcus faecalis biofilms.
Material and Methods: Fifty bovine maxillary central incisors were prepared and inoculated with E. faecalis for 60 days. The following were used as irrigation solutions: 17% EDTA (pH 3, 7 and 10), 2.5% sodium hypochlorite (NaOCl) combined with 17% EDTA (pH 3, 7 and 10), distilled water (pH 3, 7 and 10), and 2.5% NaOCl. Each solution was kept in the root canal for five minutes. Fifteen uncontaminated root canals were irrigated with 17% EDTA (pH 3, 7 and 10). Six teeth were used as bacterial control. The number of calcium, iron, manganese and zinc ions was determined using flame atomic absorption spectrometry. Mean ± standard deviation (SD) values were used for descriptive statistics.
Results: Calcium chelation using 17% EDTA at pH 7 was higher than at pH 3 and 10, regardless of whether bacterial biofilm was present. The highest concentration of iron occurred at pH 3 in the presence of bacterial biofilm. The highest concentration of manganese found was 2.5% NaOCl and 17% EDTA at pH 7 in the presence of bacterial biofilm. Zinc levels were not detectable.
Conclusions: The pH of chelating agents affected the removal of calcium, iron, and manganese ions. The concentration of iron ions in root canals with bacterial biofilm was higher after the use of 17% EDTA at pH 3 than after the use of the other solutions at all pH levels.
Recommended Citation
Estrela, C.,
Costa E Silva, R.,
Urban, R. C.,
Gonçalves, P. J.,
Silva, J. A.,
Pecora, J. D.,
&
Peters, O. A.
(2018).
Demetallization of Enterococcus faecalis Biofilm: A preliminary study.
Journal of Applied Oral Science, 26, e20170374.
DOI: 10.1590/1678-7757-2017-0374
https://scholarlycommons.pacific.edu/dugoni-facarticles/422
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Comments
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.