A hydrogel scaffold that maintains viability and supports differentiation of dental pulp stem cells
ORCiD
Dr. Benjamin D. Zeitlin: 0000-0003-0110-0188
Department
Biomedical Sciences
Document Type
Article
Publication Title
Dental Materials
ISSN
0109-5641
Volume
29
Issue
1
DOI
10.1016/j.dental.2012.08.002
First Page
97
Last Page
102
Publication Date
Winter 1-1-2013
Abstract
Objectives: The clinical translation of stem cell-based Regenerative Endodontics demands further development of suitable injectable scaffolds. Puramatrix™ is a defined, self-assembling peptide hydrogel which instantaneously polymerizes under normal physiological conditions. Here, we assessed the compatibility of Puramatrix™ with dental pulp stem cell (DPSC) growth and differentiation. Methods: DPSC cells were grown in 0.05–0.25% Puramatrix™. Cell viability was measured colorimetrically using the WST-1 assay. Cell morphology was observed in 3D modeling using confocal microscopy. In addition, we used the human tooth slice model with Puramatrix™ to verify DPSC differentiation into odontoblast-like cells, as measured by expression of DSPP and DMP-1. Results: DPSC survived and proliferated in Puramatrix™ for at least three weeks in culture. Confocal microscopy revealed that cells seeded in Puramatrix™ presented morphological features of healthy cells, and some cells exhibited cytoplasmic elongations. Notably, after 21 days in tooth slices containing Puramatrix™, DPSC cells expressed DMP-1 and DSPP, putative markers of odontoblastic differentiation.
Recommended Citation
Cavalcanti, B. N.,
Zeitlin, B. D.,
&
Nör, J. E.
(2013).
A hydrogel scaffold that maintains viability and supports differentiation of dental pulp stem cells.
Dental Materials, 29(1), 97–102.
DOI: 10.1016/j.dental.2012.08.002
https://scholarlycommons.pacific.edu/dugoni-facarticles/377