Effects of two calcium silicate cements on cell viability, angiogenic growth factor release and related gene expression in stem cells from the apical papilla.

Authors

Ove A. Peters, University of the PacificFollow
Johnah C. Galicia
Ana Arias
M. Tolar
E. Ng
S. J. Shin

ORCiD

Dr. Ove A. Peters: 0000-0001-5222-8718

Department

Endodontics

Document Type

Article

Publication Title

International Endodontic Journal

ISSN

1365-2591

Volume

49

Issue

12

DOI

10.1111/iej.12571

First Page

1132

Last Page

1140

Publication Date

12-1-2016

Abstract

AIM: To evaluate the effects of two types of calcium silicate cements on viability, angiogenic growth factor release, and angiogenic and inflammation-related gene expression in human stem cells from the apical papilla (SCAP).

METHODOLOGY: SCAPs were grown for 7 days with either ProRoot mineral trioxide aggregate (MTA) or Biodentine (BD). Cell viability and media concentrations of vascular endothelial growth factor (VEGF/VEGFA) and angiopoietin 1 (ANGPT1) were measured. The expression of genes related to angiogenic potential and inflammatory response was measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). One-way and two-way analyses of variance with multiple comparisons Tukey's test were performed (P < 0.05).

RESULTS: Cells in contact with either cement were associated with increased cell viability compared with the no-treatment group at day 1 but there were no differences amongst groups at days 3 and 7. Exposure to either cement significantly increased VEGF concentrations at day 3; however, ANGPT-1 levels decreased significantly compared with the no-treatment group at day 3. Exposure to MTA and BD stimulated expression of VEGFA and FIGF/VEGFD. Furthermore, exposure to both cements significantly decreased the mRNA levels of ANGPT1 and FGF2 relative to the no-treatment group.

CONCLUSIONS: Both MTA and BD stimulated the expression of angiogenic genes and release of VEGF, inducing similar expression patterns; however, they appeared to inhibit the expression of specific genes, including ANGPT1 and FGF2.

Recommended Citation

Peters, O. A., Galicia, J. C., Arias, A., Tolar, M., Ng, E., & Shin, S. J. (2016). Effects of two calcium silicate cements on cell viability, angiogenic growth factor release and related gene expression in stem cells from the apical papilla.. International Endodontic Journal, 49(12), 1132–1140. DOI: 10.1111/iej.12571
https://scholarlycommons.pacific.edu/dugoni-facarticles/362