Dentin inhibits the antibacterial effect of new and conventional endodontic irrigants.
ORCiD
Dr. Ove A. Peters: 0000-0001-5222-8718
Department
Endodontics
Document Type
Article
Publication Title
Journal of Endodontics
ISSN
0099-2399
Volume
39
Issue
3
DOI
10.1016/j.joen.2012.10.028
First Page
406
Last Page
410
Publication Date
3-1-2013
Abstract
INTRODUCTION: This in vitro study aimed to compare the antibacterial effect of a new irrigant, QMiX, with that of conventional irrigation solutions in the presence or absence of dentin powder.
METHODS: Dentin powder was prepared from bovine incisors and sterilized. The following irrigants were tested against Enterococcus faecalis (ATCC 29212): 6% sodium hypochlorite (NaOCl), 1% NaOCl, QMiX, 2% chlorhexidine gluconate (CHX), and 17% EDTA. Sterilized saline solution was used as negative control. Survival of bacteria exposed to the irrigants in the presence or absence of dentin was monitored under planktonic conditions. Colony-forming units were counted, and log-transformed numbers were analyzed by using Kruskal-Wallis and Mann-Whitney tests. P values less than .05 were considered significant.
RESULTS: In the absence of dentin, after 10 seconds of contact with the bacterial suspension, 6% NaOCl showed the lowest bacterial count; the difference to the negative control was significant. After 30 seconds, 6% NaOCl displayed 0 colony-forming units per milliliter, whereas 1% NaOCl and QMiX showed reduced number of colonies in comparison with the negative control. After 1 minute, both concentrations of NaOCl presented no bacterial growth and QMiX reduced the number of colonies, but EDTA and CHX had bacterial counts similar to the negative control. Dentin had a significant inhibitory effect on 6% NaOCl (10 seconds), 1% NaOCl (10 seconds and 1 minute), and QMiX (10 seconds and 1 minute). After 6 hours, both concentrations of NaOCl, QMiX, and CHX killed all bacteria, regardless of the presence of dentin.
CONCLUSIONS: Six percent NaOCl was the most effective irrigant against E. faecalis. Saline and EDTA had no measurable antibacterial effect. Dentin delayed the antibacterial activity of NaOCl and QMiX but did not completely prevent their action.
Recommended Citation
Morgental, R. D.,
Singh, A.,
Sappal, H.,
Kopper, P. M.,
Vier-Pelisser, F. V.,
&
Peters, O. A.
(2013).
Dentin inhibits the antibacterial effect of new and conventional endodontic irrigants..
Journal of Endodontics, 39(3), 406–410.
DOI: 10.1016/j.joen.2012.10.028
https://scholarlycommons.pacific.edu/dugoni-facarticles/335