ORCiD
David M. Ojcius: 0000-0003-1461-4495
Department
Biomedical Sciences
Document Type
Article
Publication Title
PLoS One
ISSN
1932-6203
Volume
9
Issue
6
DOI
10.1371/journal.pone.0099412
First Page
1
Last Page
14
Publication Date
6-23-2014
Abstract
Cancer stem cells (CSCs) represent a unique sub-population of tumor cells with the ability to initiate tumor growth and sustain self-renewal. Although CSC biomarkers have been described for various tumors, only a few markers have been identified for nasopharyngeal carcinoma (NPC). In this study, we show that CD24+ cells isolated from human NPC cell lines express stem cell genes (Sox2, Oct4, Nanog, Bmi-1, and Rex-1), and show activation of the Wnt/β-catenin signaling pathway. CD24+ cells possess typical CSC characteristics that include enhanced cell proliferation, increased colony and sphere formation, maintenance of cell differentiation potential in prolonged culture, and enhanced resistance to chemotherapeutic drugs. Notably, CD24+ cells produce tumors following inoculation of as few as 500 cells in immunodeficient NOD/SCID mice. CD24+ cells further show increased invasion ability in vitro, which correlates with enhanced expression of matrix metalloproteinase 2 and 9. In summary, our results suggest that CD24 represents a novel CSC biomarker in NPC.
Recommended Citation
Yang, C.,
Wang, H.,
Lin, Y.,
Shravan Kumar, K. P.,
Lin, H.,
Chang, C.,
Lu, C.,
Huang, T.,
Martel, J.,
Ojcius, D. M.,
Chang, Y.,
Young, J. D.,
&
Lai, H.
(2014).
Identification of CD24 as a cancer stem cell marker in human nasopharyngeal carcinoma.
PLoS One, 9(6), 1–14.
DOI: 10.1371/journal.pone.0099412
https://scholarlycommons.pacific.edu/dugoni-facarticles/13
Included in
Biochemistry Commons, Immunity Commons, Immunology of Infectious Disease Commons, Medical Immunology Commons
Comments
Article e99412