Influence of Filler Content and Polishing on Candida and Streptococci Biofilm Formation in Resin-Based Composites: An In Vitro Evaluation

Authors

• Zahra Zareshahrabadi, Basic Sciences in Infectious Diseases Research Center (SUMS)
• Sarina Sahmeddini, University of the Pacific Arthur A. Dugoni School of DentistryFollow
• Marzieh Meimandinezhad, Islamic Azad University, Shiraz Branch
• Afsoon Tondari, Islamic Azad University, Shiraz Branch
• Kamiar Zomorodian, Basic Sciences in Infectious Diseases Research Center (SUMS)

Document Type

Article

Publication Title

Canadian Journal of Infectious Diseases and Medical Microbiology

Department

Dentistry

ISSN

19181493

Volume

2025

Issue

1

DOI

10.1155/cjid/5734405

First Page

1

Last Page

13

Publication Date

6-9-2025

Abstract

Aim: The purpose of this study was to investigate the impact of filler content and polishing of resin-based composites on in vitro biofilm formation of Candida and Streptococci species. Materials and Methods: Specimens of four commercially available resin-based composites including Z100, P60, Z250, and Z350, with different filler amounts and volumes, were prepared according to the manufacturer’s instructions. Each group was divided into polished and unpolished specimens, which were then placed in a 24-well tissue culture plate with microbial suspension and incubated. The XTT technique was used to evaluate biofilm formation. Results: Z250 resin-based composites, which had the highest percentage of filler (68%), had the highest biofilm metabolic activity. A significantly less microbial biofilm metabolic activity was noted on P60 polished resin-based composites than on unpolished groups (p < 0.001). Polishing procedures reduce biofilm metabolic activity. Streptococcus salivarius produced the least biofilm metabolic activity among the Streptococcal species (p < 0.001). However, there were no statistically significant differences between Candida species in the biofilm metabolic activity. Conclusion: The results revealed that the amount of filler in resin-based composites had a major impact on the biofilm metabolic activity. Therefore, resin-based composites with a minimized excess resin matrix, minimized filler amount, and smoother surfaces might be more useful in reducing biofilm metabolic activity and secondary caries. These findings may be useful for modifying novel resin-based composite formulations to improve oral health and patient wellbeing.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

Zareshahrabadi, Zahra; Sahmeddini, Sarina; Meimandinezhad, Marzieh; Tondari, Afsoon; and Zomorodian, Kamiar, "Influence of Filler Content and Polishing on Candida and Streptococci Biofilm Formation in Resin-Based Composites: An In Vitro Evaluation" (2025). Pacific Faculty Work. 35.
https://scholarlycommons.pacific.edu/all-faculty/35