Document Type
Article
Publication Title
International Journal of Molecular Sciences
Department
Chemistry
ISSN
14220067
Volume
25
Issue
23
DOI
10.3390/ijms252312617
First Page
1
Last Page
13
Publication Date
11-24-2024
Abstract
Consisting of more than 11,000 members distributed over five families, the tautomerase superfamily (TSF) is a large collection of proteins with diverse biological functions. While much attention has been given to individual TSF enzymes, a majority remain structurally and functionally uncharacterized. Given its large size, studying a representative member of each family offers a viable approach for extracting mechanistic insights applicable to the entire superfamily. In this study, cis-3-chloroacrylic acid dehalogenase (cis-CaaD), 5-carboxymethyl-2-hydroxymuconate isomerase (CHMI), malonate semialdehyde decarboxylase (MSAD), and 4-oxalocrotonate tautomerase (4-OT) were referenced against the well-studied macrophage migration inhibitory factor (MIF) and D-dopachrome tautomerase (D-DT) using triplicate 1 μs molecular dynamics (MD) simulations for a total of 18 μs. Through root mean square fluctuation (RMSF) measurements, correlation analyses, and comparisons to previous crystallographic structures, we reveal key mechanistic insights that promote the understanding of the catalytic activities in TSF. Collectively, our findings from these functionally diverse TSF proteins provide key information on allosteric coupling, long-range intra- and inter-subunit communications as well as structure–activity relationships that enable new studies in the superfamily.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Argueta, Christopher; Parkins, Andrew; and Pantouris, Georgios, "Conformational Flexibility of the C-Terminal Region Influences Distal Active Site Residues Across the Tautomerase Superfamily" (2024). Pacific Faculty Work. 26.
https://scholarlycommons.pacific.edu/all-faculty/26
