Document Type
Article
Publication Title
International Journal of Molecular Sciences
Department
Biomedical Sciences
ISSN
14220067
Volume
25
Issue
12
DOI
10.3390/ijms25126382
First Page
1
Last Page
7
Publication Date
6-9-2024
Abstract
SARS-CoV-2 S-protein-mediated fusion is thought to involve the interaction of the membrane-distal or N-terminal heptad repeat (NHR) (“HR1”) of the cleaved S2 segment of the protein and the membrane-proximal or C-terminal heptad repeat (CHR) (“HR2”) regions of the protein. We examined the fusion inhibitory activity of a PEGylated HR2-derived peptide and its palmitoylated derivative using a pseudovirus infection assay. The latter peptide caused a 76% reduction in fusion activity at 10 µM. Our results suggest that small variations in peptide derivatization and differences in the membrane composition of pseudovirus preparations may affect the inhibitory potency of HR2-derived peptides. We suggest that future studies on the inhibition of infectivity of SARS-CoV-2 in both in vitro and in vivo systems consider the need for higher concentrations of peptide inhibitors.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Düzgüneş, Nejat; Tao, Zhihua; Zhang, Yuxia; and Krajewski, Krzysztof, "Peptides Derived from the SARS-CoV-2 S2-Protein Heptad-Repeat-2 Inhibit Pseudoviral Fusion at Micromolar Concentrations: The Role of Palmitic Acid Conjugation" (2024). Pacific Faculty Work. 24.
https://scholarlycommons.pacific.edu/all-faculty/24
