Role of TRAIL-R in Primary and Secondary Genital and Respiratory Chlamydia muridarum Infections in Mice

Authors

• Sukumar Pal, UCI School of Medicine
• Sydni Sheff, UCI School of Medicine
• Mufadhal Al-Kuhlani, University of the PacificFollow
• David M. Ojcius, University of the PacificFollow
• Luis M. de la Maza, UCI School of Medicine

Document Type

Article

Publication Title

Microbiology Spectrum

Department

Biomedical Sciences

ISSN

21650497

Volume

10

Issue

4

DOI

10.1128/spectrum.01617-22

First Page

1

Last Page

11

Publication Date

7-25-2022

Abstract

The tumor necrosis factor (TNF)-related apoptosis-inducing ligand receptor (TRAIL-R) suppresses inflammation and could therefore affect the course of Chlamydia infections and their long-term sequelae. Wild-type (WT) and TRAIL-R^2/2 C57BL/6 mice were inoculated vaginally with Chlamydia muridarum; the course of the infection was followed with vaginal cultures and the presence of hydrosalpinx determined. To evaluate the role of TRAIL-R following a secondary infection, the mice were vaginally reinfected. WT and TRAIL-R^2/2 male mice were also infected and reinfected in the respiratory tract, and the course of the diseases and the infections were followed. Following the primary and secondary vaginal infection, no significant differences in vaginal shedding or hydrosalpinx formation were observed between the WT and TRAIL-R^2/2 mice. The WT and TRAIL-R^2/2 mice mounted antibody responses in serum and vaginal washes that were not significantly different. After the primary and secondary intranasal infections of the male mice, changes in body weight were determined, and no significant differences were observed between the WT and TRAIL-R^2/2 mice. Ten days after the primary and the secondary infections, the weight of the lungs and number of C. muridarum inclusion forming units (IFU) were determined. The lungs of the WT mice weighed less compared with the TRAIL-R^2/2 mice following a primary infection but not after a secondary infection. No differences in the number of C. muridarum IFU in the lungs were observed between the two groups of mice. In conclusion, despite playing a role in inflammation cell-signaling pathways in vitro, TRAIL-R does not appear to play a major role in the susceptibility, clinical outcomes, or long-term sequelae of C. muridarum infections in vivo.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

Pal, Sukumar; Sheff, Sydni; Al-Kuhlani, Mufadhal; Ojcius, David M.; and de la Maza, Luis M., "Role of TRAIL-R in Primary and Secondary Genital and Respiratory Chlamydia muridarum Infections in Mice" (2022). Pacific Faculty Work. 220.
https://scholarlycommons.pacific.edu/all-faculty/220