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Date of Award

2015

Document Type

Thesis - Pacific Access Restricted

Degree Name

Master of Science (M.S.)

Department

Biological Sciences

First Advisor

Lisa Wrischnik

First Committee Member

William Chan

Second Committee Member

Douglas Weiser

Abstract

The aryl hydrocarbon receptor (AhR) carboxyl terminal transcriptional activation domain was cloned, purified in denatured conditions from bacteria, refolded via limited dialysis, and analyzed for proper refolding via co-immunoprecipitation with the known binding partner SRC-1. This AhR NΔ515 transactivation domain construct was used, along with amino terminal AhR deletion constructs AhR CΔ274 and AhR CΔ553, to attempt to elucidate the nature of the interaction between AhR and p23 in vitro.

Pages

101

ISBN

9781339047355

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