Title

LUCIFERASE GENE TRANSFECTION MEDIATED BY CATIONIC LIPOSOMES COMPRISING NOVEL TRANS-2-AMINOCYCLOHEXANOL-BASED AMPHIPHILES

Lead Author Affiliation

Chemistry

Second Author Affiliation

Chemistry

Third Author Affiliation

Chemistry

Fourth Author Affiliation

Chemistry

Fifth Author Affiliation

Chemistry

Sixth Author Affiliation

Department of Pharmaceutics and Medicinal Chemistry

Introduction

Gene transfection is one of the most promising and rapidly developing methodologies in modern medicine. Gene material (DNA) is commonly delivered into cells in a form of complexes with liposomes (lipoplexes).

Purpose

Development of a novel type of acid-sensitive liposomes for targeted gene delivery.

Method

We suggested recently a novel type of pH-sensitive drug delivery system, “fliposomes”, which contain trans-2-aminocyclohexanol-based amphiphiles, “flipids”, that perform a conformational flip (a drastic change of molecular shape) and trigger an instant cargo release in acidic media. The present research has explored a possibility of application of fliposomes in gene delivery.

Results

We found that incorporation of trans-2-aminocyclohexanol-based amphiphiles as helper lipids in DOTAP-cationic liposomes enhances substantially the transfection of the Gaussia luciferase plasmid into HeLa and B16F1 cells. The efficiency of transfection strongly depends on the structure of lipophilic chains and NRR’ groups.

Significance

New type of acid-sensitive liposomes provides a prospective gene delivery system for application in biology and medicine.

Location

DeRosa University Center, Stockton campus, University of the Pacific

Format

Poster Presentation

This document is currently not available here.

Share

COinS
 
Mar 25th, 10:00 AM Mar 25th, 3:00 PM

LUCIFERASE GENE TRANSFECTION MEDIATED BY CATIONIC LIPOSOMES COMPRISING NOVEL TRANS-2-AMINOCYCLOHEXANOL-BASED AMPHIPHILES

DeRosa University Center, Stockton campus, University of the Pacific

Gene transfection is one of the most promising and rapidly developing methodologies in modern medicine. Gene material (DNA) is commonly delivered into cells in a form of complexes with liposomes (lipoplexes).