Title

177390 ALOX12/15 AND COX2 IN HUMAN BM-MSC AND SCAP IN VITRO

Lead Author Affiliation

Dugoni School of Dentistry

Second Author Affiliation

Dugoni School of Dentistry

Third Author Affiliation

Dugoni School of Dentistry

Fourth Author Affiliation

Dugoni School of Dentistry

Fifth Author Affiliation

Dugoni School of Dentistry

Introduction

Traditionally, human bone marrow-derived and mesenchymal stem cells from the dental apical papilla (BM-MSC and SCAP, respectively) are cultivated in normal atmospheric level of oxygen (21%). Their isolation and transfer to culture represents a shift from 2-8% in their niche to 21% in culture. Their re-implantation would mean a shift from 21% to 2-8% in the implantation site.

Purpose

Does the change from 21% to 5% oxygen influence physiological status of mesenchymal stem cells?

Method

Human BM-MSC and SCAP were freshly isolated (IRB approval Nr.09-99.1). Cells were grown in basal medium from Lonza, 10% human adult serum, 2mM L-glutamin and antibiotics. Gene expression of COX2, ALOX 12 and 15 was measured by RT PCR (SABiosciences, Applied Biosystems) following 21 to 5% and 5 to 21% transfers.

Results

Following 5 to 21% transfer, no significant change in gene expression was observed. Transfer 21 to 5% led to increased expression of COX2 in BM-MSC. Transfer 21 to 5% led to increased expression of ALOX12 and 15 in SCAP.

Significance

The increase in ALOX12/15 and COX2 gene expression may be a part of a cellular reaction to hypoxia. We recommend that a tissue level of oxygen is maintained during isolation and expansion of human BM-MSC and SCAP in vitro.

Location

DeRosa University Center, Stockton campus, University of the Pacific

Format

Poster Presentation

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Mar 25th, 10:00 AM Mar 25th, 3:00 PM

177390 ALOX12/15 AND COX2 IN HUMAN BM-MSC AND SCAP IN VITRO

DeRosa University Center, Stockton campus, University of the Pacific

Traditionally, human bone marrow-derived and mesenchymal stem cells from the dental apical papilla (BM-MSC and SCAP, respectively) are cultivated in normal atmospheric level of oxygen (21%). Their isolation and transfer to culture represents a shift from 2-8% in their niche to 21% in culture. Their re-implantation would mean a shift from 21% to 2-8% in the implantation site.