Campus Access Only
All rights reserved. This publication is intended for use solely by faculty, students, and staff of University of the Pacific. No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, now known or later developed, including but not limited to photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author or the publisher.
Date of Award
Thesis - Pacific Access Restricted
Master of Science (M.S.)
First Committee Member
Lisa A. Wrischnik
Second Committee Member
Third Committee Member
Fourth Committee Member
The aim of this project was to study the population genetics of Echinostoma trivolvis, a parasitic trematode that uses multiple hosts in its lifecycle and has a significant impact on amphibian populations. Microsatellite markers were to be identified and isolated because of their highly variable nature and reported ease of use with PCR. Parasite DNA was extracted from planorbid snails from several locations within California including: Point Reyes National Seashore, Lake Tahoe, and the San Francisco Bay Area. In addition, parasite samples were obtained from Manitoba, Canada. Several microsatellites were identified and 29 PCR primers sets were designed, six of which were capable of amplifying consistently. Sequencing other published molecular markers, COl, NDl, and ITS, unveiled intriguing phylogenetic relationships and potential cryptic species. The echinostome population in central California, as a result of this project, may be much more diverse than has long been reported in the literature.
Butcher, Bradley J.. (2010). Identification and isolation of microsatellite loci from the Trematode Echinostoma Trivolvis for use in interspecific and intraspecific variation studies. University of the Pacific, Thesis - Pacific Access Restricted. https://scholarlycommons.pacific.edu/uop_etds/750
To access this thesis/dissertation you must have a valid pacific.edu email address and log-in to Scholarly Commons.Find in PacificSearch
If you are the author and would like to grant permission to make your work openly accessible, please email
In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/
This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).