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Date of Award
Thesis - Pacific Access Restricted
Master of Science (M.S.)
First Committee Member
Second Committee Member
Third Committee Member
Craig A. Vierra
In the fission yeast S. pombe, Mto1p and Mto2p are involved in MT nucleation from cytoplasmic MTOCs and recruit components of the γ-TuC. We wished to discover whether Ben1p plays a similar role. ben1+ was tagged at its normal chromosomal location and the intracellular localization was analyzed. It showed that Ben1p localized to cytoplasmic microtubules. We generated ben1Δ. cells by replacing it with a copy of ura4+. We observed that 20% of the cells were undergoing division. Next we observed the effects that deleting ben1+ would have on the functions of Mto1p and Mto2p. We setup crosses between these strains and observed their localization. We did not observe any specific Mto2-GFP localization in ben1Δ. cells. This suggests that Ben1p plays a role in the proper localization of a γ-TuC associated protein.
Escotto, Benjamin Alan. (2009). Identification and characterization of BEN1, a novel microtubule associated protein in fission yeast. University of the Pacific, Thesis - Pacific Access Restricted. https://scholarlycommons.pacific.edu/uop_etds/715
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