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Date of Award
Thesis - Pacific Access Restricted
Master of Science (M.S.)
Lisa A. Wrischnik
First Committee Member
Second Committee Member
Gregg D. Jongeward
T. vaginalis is a protozoan parasite without an observed sexual stage in its life cycle. However, T. vaginalis has genes, such as Dmc1, known to be involved in meiosis in other organisms. In order to look at the expression of these genes in T. vaginalis, RT-PCR was done using purified mRNA. It shows that Dmc1 is expressed in both normal and drug treated cells. However, relative levels are unclear. Localization studies were done in T. vaginalis using immunofluorescence against Dmc1 protein with an HA tag. These studies showed that recombinant Dmc1 remained in the cytoplasm upon treatment with DNA damaging drugs. Additionally, T.vaginalis Dmc1 protein was expressed and purified from E.coli to have polyclonal antibodies made to use in further immunofluorescence studies.
Fullerton, Donna Lynn. (2007). Investigation of the expression of DMC1, a meiotic gene, in trichomonas vaginalis. University of the Pacific, Thesis - Pacific Access Restricted. https://scholarlycommons.pacific.edu/uop_etds/664
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