Campus Access Only

All rights reserved. This publication is intended for use solely by faculty, students, and staff of University of the Pacific. No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, now known or later developed, including but not limited to photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author or the publisher.

Date of Award


Document Type

Thesis - Pacific Access Restricted

Degree Name

Master of Science (M.S.)


Biological Sciences

First Advisor

Douglas C. Weiser

First Committee Member

Craig A. Vierra

Second Committee Member

Douglas Risser


Regulation of smooth muscle contraction depends on the phosphorylated state of myosin light chain (MLC). Although there are many kinases responsible for phosphorylating MLC, the myosin phosphatase complex is solely accountable for its dephosphorylation. Myosin phosphatase, in turn, is tightly regulated by many proteins. One of them being the CPI-17 gene family, which inhibits myosin phosphatase. This family of proteins is composed of CPI-17 itself, PHI-1, KEPI, and GBPI. Zebrafish have two genes each of CPI-17 and PHI-1, which are expressed during early embryonic development. This study sets out to investigate whether the two isoforms of CPI-17 and PHI-1 have diverged in function or expression using zebrafish as a model organism. Through a series of biochemical tests and assays, we have determined that the two isoforms have diverged in their expression pattern from each other, however they have similar function.



To access this thesis/dissertation you must have a valid email address and log-in to Scholarly Commons.



If you are the author and would like to grant permission to make your work openly accessible, please email


Rights Statement

Rights Statement

In Copyright. URI:
This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).