Campus Access Only

All rights reserved. This publication is intended for use solely by faculty, students, and staff of University of the Pacific. No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, now known or later developed, including but not limited to photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author or the publisher.

Date of Award


Document Type


Degree Name

Master of Science (M.S.)


Graduate Studies

First Advisor

James Blankenship

First Committee Member

Marvin H. Malone

Second Committee Member

Katherine K. Knapp

Third Committee Member

Herschel Frye


An enzyme activity located in the soluble fraction of rat liver which is known to deacetylate N8-acetylspermidine is studied. Using a crude cytosol preparation from rat liver, tritium-labelled N8-acetylspermidine [acetyl-3H] was shown to undergo deacetylation which was dependent upon both time of incubation and protein concentration. The Michalis constant for this deacetylation of N8-acetylspermidine is approximately 4.4 μM.

N1-Acetylspermidine showed a competitive inhibition of this N8-acetylspermidine deacetylation activity, and was found to be the most potent inhibitor tested. Diamine and polyamine compounds were also shown to inhibit the deacetylation of N8-acetylspermidine. Spermidine was the most potent inhibitor of the naturally occurring polyamines tested, followed in order by spermine, putrescine, and cadaverine. Of numerous acetylated compounds studied, only acetylprocainamide showed any inhibition of this N8-acetylspermidine deacetylating activity at concentrations below 10.0 mM.

The possible functions of the deacetylating activity are discussed along the speculatio of the role this enzyme activity may play in vivo.



Included in

Chemistry Commons



Rights Statement

Rights Statement

No Known Copyright. URI:
The organization that has made the Item available reasonably believes that the Item is not restricted by copyright or related rights, but a conclusive determination could not be made. Please refer to the organization that has made the Item available for more information. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use.