Campus Access Only

All rights reserved. This publication is intended for use solely by faculty, students, and staff of University of the Pacific. No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, now known or later developed, including but not limited to photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author or the publisher.

Date of Award


Document Type


Degree Name

Master of Science (M.S.)


Graduate School

First Advisor

Herschel Frye

First Committee Member

Richard P. Dodge

Second Committee Member

Emerson G. Cobb


In recent years elevated levels of serum triglycerides have become of increasing importance in the field of medical technology. Abnormally high triglycerides have been claimed to be a major cause of numerous diseases and illnesses. Fredrickson and his associates (7, 15) have introduced a system for classifying hyperlipidemia and in all classes elevated triglycerides is a major laboratory finding. Disorders include obesity, diabetes, pancreatitis, xanthomatosis, hypothyroidism, and liver and kidney diseases; but most important is atherosclerosis

Some work has been done in the development of new methods of serum triglyceride analyses. The most advanced work makes use of an "automated analyzer" of the type produced by Technicon Corporation under the trademark AutoAnalyzer. This instrument determines the serum levels by the same method as above but at a faster rate, but the equipment is costly. A second method makes use of light scattering indices (nephelometry) to assess serum triglycerides. Work in this area has been done by Helman, Blevins, and Gleason (12). Their results were consistent, in most cases, with those of the colorimetric method. Of the thirty Fredrickson classifications they made by nephelometry twenty-one were in agreement with manual methods. However, Baty and Batsakis (1) have concluded that nephelometry provides too indirect an assay to give consistent results for serum triglycerides. A third method employs chromatography and infrared spectrophotometry. Freeman, Lindgren, Ng, and Nichols (8) showed that first, the lipids could be separated by chromatographic techniques, and then the extraction could be analyzed by infrared measurements. However, it is found that fractionating the lipid leads to error and is quite time consuming. Still other methods use phenylhydrazine (13, 16), mercaptoacetic acid (5), or oxidation to yield aldehydes, which are then thin-layer chromatographed (18).

In light of the numerous above methods and the error and time involved in analysis, the development of a new improved method with the time factor in mind was attempted. The use of infrared spectrophotometry was employed, but without the use of any prior extractions. It is hoped that this technique can give accurate and consistent values for triglyceride levels with a minimum amount of time expended. The new method should also be easy to install with a simplified procedure which would minimize error.



Included in

Chemistry Commons



Rights Statement

Rights Statement

No Known Copyright. URI:
The organization that has made the Item available reasonably believes that the Item is not restricted by copyright or related rights, but a conclusive determination could not be made. Please refer to the organization that has made the Item available for more information. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use.