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Date of Award


Document Type


Degree Name

Master of Arts (M.A.)



First Advisor

Donald L. Lehmann


The in vitro cultivation of Trypanosome ranarum has been accomplished in complex nutrient solutions, but growth in chemically defined media has never been attempted. The purpose of the study was to cultivate R. ranarum in “purified” bacterial media ingredients and to determine the basic nutrient requirements of the organism. Preliminary studies were also undertaken to determine the effects of environmental conditions upon the cultural flagellates and in addition, morphological studies were made on liquid forms as well as colonies from solid media.



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